Listing of all reported Splice Mutations in the FVIII Gene 
08-May-12
Please note that aminoacid numbering given below is for the mature processed protein, as used almost universally in FVIII studies. 
To convert to HGVS-type aminoacid numbering (where initiator Met =+1) add 19 to aminoacid numbers.     
Nucleotide numbering where given should conform to the convention that "A" in initiation codon ATG = +1  
Recommended syntax for sequence variations may be seen at   
http://www.genomic.unimelb.edu.au/mdi/mutnomen/          
Exon/Intron Aminoacid Nucleotide Change Aminoacid Change FVIII:C (%) FVIII:Ag (%) Severity Inhibitors Comments References Date added to HADB
1 Intron a>g - - ? ? Severe ? New donor splice ~1.3KB 3' of exon1 Western et al (unpublished) Aug-05
1 Intron a>g - - 2 ? Moderate No New donor splice 1.4KB 3' of exon1 Bagnall et al (1999) Feb-00
1 Intron a>g - - <1 ? Moderate No New donor splice 1.4KB 3' of exon1 Bagnall et al (1999) Feb-00
1 Intron IVS1+2T>C - - 21 18 Mild No IVS1+2T>C Casana et al (2004) Jul-07
1 29 AGA>AAA Arg Lys <1 ? Severe No -1 IVS1 acceptor splice site Becker et al (1996) Aug-96
1 In1/Ex2 GA>AA ? ? 2 ? Severe No -1 IVS1 acceptor splice site Timur et al (2001) Oct-01
1 In1/Ex2 a>g ? ? <1 ? Severe No -2bp ex2 acceptor splice Liu et al (2002) Mar-02
1 Intron (-26) A>T c.-143 - - <1 ? Severe No (-26) A>T c.-143 Jayandharan et al (2005) Aug-07
2 Intron tcttcacgcag/A>gcttcacgcag/A - - 3-5 ? ? No - Lillicrap et al (unpublished) Oct-02
2 Intron G/g>G/a - - 2 45 Severe No Donor splice intron 2 Liu et al (1998) May-99
2 Intron cga>tga - - <1 ? Severe No 300bp 3' to exon 2 Gitschier et al (1985) Dec-85
2 Intron cag>caa - - 2 ? Severe No Acceptor splice Timur et al (2001) Oct-01
2 Intron G265+1A - - <1 ? Severe No Splice donor Goodeve et al (2000) Feb-01
3 Intron c.294-2A>T - - 1 ? Severe ? -3 IVS3  Ahmed et al (2005) Aug-05
3 Intron c>t - - 31 ? Mild No -9 IVS3 also Val1857Leu Timur et al (2001) Oct-01
3 70 GGT>GAT Gly Asp <1 ? Severe No -1 IVS2 donor splice site Becker et al (1996) Aug-96
3 Intron g>a - - ? ? Severe No 5nt past end exon 3 Klopp et al (2002) Dec-02
3 Intron AGG/gtgag>AGG/gtgaa - - <1 ? Severe No IVS3+5 donor splice site Fidalgo et al (unpublished) Aug-04
3 Intron  IVS3+5G>A - - <1 ? Severe No IVS3+5 donor splice site Riccardi et al (unpublished) Nov-05
3 Intron AG/>TG/ - - <1 ? Severe Yes -2 IVS3 acceptor splice site Riccardi et al (unpublished) Jan-04
4 Intron c.601+5G>A - - 9 ? Mild ? +5 IVS4 Ahmed et al (2005) Aug-05
4 Intron cga>caa - - 5-10 41 Mild No May activate cryptic splice Youssouffian et al (1988c) Dec-88
4 Intron tctttttcag/GG>gctttttcag/GG - - 7 ? Mild No splicing error Gallardo et al (unpublished) Nov-06
4 Intron ag/GG>aa/GG - - <1 ? Severe No  -1 IVS4 acceptor splice site Fernandez-Lopez et al (2005) Jul-07
4 Intron ag/>gg/ - - 2 1 Severe No -2 IVS4 acceptor splice site David et al (1994) Dec-94
5 Intron nt6 T>C - - 3 ? ? ? IVS 5 Ggttagtga>Ggttagcga Chan et al (1996) Dec-96
5 Intron nt6 T>C - - 10 ? ? ? IVS 5 Ggttagtga>Ggttagcga Chan et al (1996) Dec-96
5 Intron G>A - - <1 ? ? ? IVS5 +5G>A Citron et al (2002) Jul-03
5 205 G/GG>T/GG Gly Trp 3 ? Moderate ? -1 IVS5 donor splice site Higuchi et al (1991a) Dec-91
5 Intron C>G - - <1 ? Severe ? -3 IVS5 acceptor, deletes Ex6/5&6 Tavassoli et al (1998) Jan-99
5 Intron del 12 nt actctatctttg - - 12 ? Mild No small deletion splicing error Gallardo et al (unpublished) Nov-06
5 Intron ag/>gg/ - - <1 ? Severe No -2 IVS5 acceptor splice site Naylor et al (1991) Dec-91
5 Intron A>G - - <1 ? Severe No IVS 5 670-2: also M2238V polymorphism Williams et al (1998) Jul-98
5 Intron A>G - - <1 ? Severe No IVS5 c.671-2 A>G:  Williams et al (1998) Jul-98
5 Intron G/gt>G/gg - - ? ? Severe No - Lillicrap et al (unpublished) Oct-02
5 Intron IVS5+1G>T - - <1 ? Severe No IVS5+1G>T Riccardi et al (unpublished) Nov-05
5 Intron IVS5 613+5G>A - - 4 ? Severe No IVS5 +5G>A Arnold et al (unpublished) Jul-06
6 Intron ctttacag/GT>gtttacag/GT - - ? ? Mild ? splicing error Gallardo et al (unpublished) Nov-06
6 Intron ag/>ac/ - - ? ? Severe ? -1 IVS6 acceptor splice site Antonarakis et al (unpublished) Dec-91
6 Intron ag/>at/ - - <1 ? Severe ? -1 IVS6 acceptor splice site Bicocchi et al (2003) Aug-04
6 Exon CCA>CCC Pro Pro 25 75 Mild No -2 IVS6 donor splice site ?causative Liu et al (1998) May-99
6 Intron G/gta>G/gtg - - 5 ? Moderate ? +3 IVS6 donor splice site Nesbitt et al (unpublished) Aug-05
6 Intron G/gta>G/gtg - - 3 ? Moderate No +3 IVS6 donor splice site Bidichandani et al (1994) Dec-94
6 Intron G/gta>G/gtg - - 3-4 ? Moderate No +3 IVS6 donor splice site Bidichandani et al (1994) Dec-94
6 Intron G/gta>G/gtg - - <1 ? Severe No +3 IVS6 donor splice site Theophilus et al (2001) Aug-01
6 Intron G/gta>G/gtg - - <1 <1 Severe No +3 IVS6 donor splice site Ljung et al (unpublished) Jul-07
6 Intron AG/gta>AG/ata - - <1 ? Severe No +1 IVS6 donor splice site Gallardo et al (unpublished) Nov-06
7 Intron c.1009+3A>C - - 2 ? Mild ? IVS7 +3 Ahmed et al (2005) Aug-05
7 Intron c.1009+1G>A - - <1 ? Severe ? IVS7 +1 Ahmed et al (2005) Aug-05
7 Intron ag/ATG>cg/ATG - - <1 ? Severe No IVS7-2 acceptor splice site Silva Pinto et al (unpublished) Aug-04
7 Intron TG/gta>TG/ata - - <1 ? Severe No +1 IVS7 donor splice site Gallardo et al (unpublished) Nov-06
7 Intron (-23) G>A - - <1 ? Severe No polymorphism? Jayandharan et al (2005) Aug-07
8 Intron G>A - - <1 ? Severe ? +1 IVS8 donor splice site Cutler et al (2002) May-02
9 Intron nt1 G>A - - 3 ? ? ? IVS 9 Ggtaagtt>Gataagtt Chan et al (1996)) Dec-96
9 Exon 1443G>A codon 462 Leu Leu 12 ? Mild ? 1443G>A at end exon 9 Ahmed et al (2005) Aug-05
9 Intron c.1443+5G>C - - 1 ? Moderate ? IVS 9 +5 Ahmed et al (2005) Aug-05
9 Intron c.1443+18T>G - - 1 ? Moderate ? IVS 9 +18 Ahmed et al (2005) Aug-05
9 Exon 1443G>A codon 462 Leu Leu 8 ? Mild No 1443G>A at end exon 9 Casey et al (unpublished) Aug-04
9 Intron #2 T>C - - <1 ? Severe Yes IVS 9 #2 T>C Casey et al (unpublished) Aug-04
10 Intron AG/gtaaatattc>AG/gtaaatattg - - NA carrier ? NA carrier ? splice error in carrier only Gallardo et al (unpublished) Nov-06
10 Intron c.1473-2A>G - - <1 ? Severe ? IVS 10 -2 Ahmed et al (2005) Aug-05
10 Intron ag/GT>at/GT - - <1 ? ? No acceptor site lost Habart et al (2003) Aug-05
10 Intron (-2) A>G c.1538 - - <1 ? Severe No also has IVS9 (+18) T>G (poly?) Jayandharan et al (2005) Aug-07
11 Intron nt5 G>A - - 7 ? ? ? IVS 11 Ggtgagtt>Ggtgaatt Chan et al (1996) Dec-96
11 504 CTG>CTT Leu Leu ? ? Mild ? Potential new acceptor site Diamond et al (1992) Dec-92
11 504 CTG>CTT Leu Leu 8-14 ? Mild ? Cryptic splice del 36bp Exon 11 Tavassoli et al (1998) Jan-99
11 504 CTG>CTT Leu Leu ? ? Mild No Potential new acceptor site Economou et al (1992) Dec-92
11 504 CTG>CTT Leu Leu ? ? Mild No Potential new acceptor site Economou et al (1992) Dec-92
11 504 CTG>CTT Leu Leu ? ? Mild No Potential new acceptor site Economou et al (1992) Dec-92
11 504 CTG>CTT Leu Leu 7 ? Mild No Potential new acceptor site Vidal et al (2001) Aug-01
11 565 CAG>AAG Gln Lys ? ? Mild/Moderate No /gt -3 IVS11 donor splice site Higuchi et al (1991a) Dec-91
11 565 CAG>AAG Gln Lys ? ? Moderate No /gt -3 IVS11 donor splice site Antonarakis et al (unpublished) Dec-91
11 Intron G>A - - <1 ? Severe No IVS 11 c.1753-1 G>A Bogdanova et al (2005) Jul-06
12 Intron /gtgagt>/gtgaat - - ? ? Mild ? +5 IVS12 donor splice site Antonarakis et al (unpublished) Dec-91
12 Intron A/gt>A/at - - ? ? Severe ? - Lillicrap et al (unpublished) Oct-02
12 Intron del G - - ? ? Severe ? 1903 +3 del G donor splice Nesbitt et al (unpublished) Aug-05
12 Intron agt>att - - 6 117 Mild No Ex12 +5 donor splice site ?causative Liu et al (1998) May-99
13 616 A/GC > A/AC Ser Asn 6-28 30 Mild No acceptor splice Liu et al (2002) Mar-02
13 Intron G/g>G/a - - <1 <1 Severe No -1IVS13 donor splice Ljung et al (unpublished) Jul-07
13 Intron cag>caa - - <1 7 Severe Yes Ex14 -1 acceptor splice site Liu et al (1998) May-99
14 Intron InsA - - <1 ? ? ? IVS14 -16 InsA Citron et al (2002) Jul-03
14 Exon C5217T Asn 1720 39 ? Mild ? Splice   Western et al (unpublished) Aug-05
14 Intron AG/gta>AG/gtg - - 9 ? Mild ? Splice   Nesbitt et al (unpublished) Aug-05
14 Intron ag/GTA>ag/GTG ? ? <1 <3 Severe No -3 IVS14 acceptor splice site Seehafer et al (unpublished) Dec-96
14 Intron G>A - - <1 ? Severe No IVS14-1 G>A Frusconi et al (2002) Nov-02
14 Intron G/gt>G/gg - - <1 ? Severe No - Lillicrap et al (unpublished) Aug-05
14 Intron IVS14+3A>G - - ? ? Mild ? IVS14+3A>G Lombardi et al (2004) Jul-07
15 Intron G>C - - <1 ? ? ? IVS15 +1G>C Citron et al (2002) Jul-03
15 Intron G>A - - <1 ? ? ? IVS15 +1G>A Citron et al (2002) Jul-03
15 Intron gtg>ttg - - <1 <1 Severe No Ex15 +1 donor splice site Liu et al (1998) May-99
15 Intron gtg>ttg - - <1 ? Severe No Ex15 +1 donor splice site Yenchitsomanus et al (2001) Aug-01
15 Intron cag>ctg - - <1 ? Severe No IVS15 acceptor site Tagariello et al (unpublished) Apr-00
15 Intron (+6) T>G c.5373 - - <1 ? Severe No (+6) T>G c.5373 Jayandharan et al (2005) Aug-07
16 1843 CTG>CTA Leu Leu 9-18 ? Moderate ? -1 IVS16 donor splice site Higuchi et al (1991a) Dec-91
16 Intron tag>taa - - <1 ? Severe No -1 IVS16 acceptor splice site Vidal et al (2001) Aug-01
17 Intron gca/ATC>tca/ATC - - ? ? Severe ? Int17/Ex18 Splice Nesbitt et al (unpublished) Aug-05
17 Intron t>c - - <1 ? Severe No +2 IVS17 donor splice Liu et al (2002) Mar-02
18 Intron AG/g>AG/a - - <1 ? Severe No Skip Exon 18/part Exon 19 Freson et al (1998) Apr-99
18 Intron AG/g>AG/a - - <1 ? Severe No Skip Exon 18/part Exon 19 Theophilus et al (2001) Aug-01
18 Intron AG/g>AG/a - - 2 ? Severe No Skip Exon 18/part Exon 19 Lillicrap et al (unpublished) Oct-02
18 Intron (+91) G>A - - <1 ? Severe No polymorphism? Jayandharan et al (2005) Aug-07
19 ??? Agtg>Agtt ?? ? ? Severe ? Exon 19 donor splice site Lambert et al (1997) Apr-97
19 Intron A>G - - <1 ? Severe ? IVS19 -2 Del exon 20 Tavassoli et al (1998) Jan-99
19 Intron A/gt>A/gc - - <1 ? Severe ? - Lillicrap et al (unpublished) Aug-05
19 Intron Alu element insertion - - <1 ? Severe ? exon 19 skipped Ganguly et al (2003) Jun-07
19 Intron TA/gtgagtagc>TA/gtgagtagg - - 23 ? Mild No splicing error Gallardo et al (unpublished) Nov-06
19 Intron gtgagt>gtgaat - - 1 ? Severe No Excises exon 19 Tavassoli et al (1997) Aug-97
19 Intron ATA/gtgagt>ATA/gtgaga - - <1 ? Severe No - Gallardo et al (unpublished) Sep-03
19 Intron G>A - - <1 0.3 Severe No IVS19 +1 G>A Ivaskevicius et al (2001) Aug-01
19 Intron A IVS19 +4G inserts Glu ? ? Severe No deletes exon 19 Bicocchi et al (2004); Bicocchi et al (2005B) Jul-07
21 Intron c.6273+1G>T - - <1 ? Severe ? IVS 21 +1 Ahmed et al (2005) Aug-05
22 Intron C>G - - <1 ? ? ? IVS22 -3 Citron et al (2002) Jul-03
22 Intron g>t - - ? ? Moderate ? 5nt past end exon 22 Klopp et al (2002) Dec-02
22 Intron TG/g>TG/a - - <1 ? Severe No - Lillicrap et al (unpublished) Oct-02
22 Intron t>a - - ? ? Severe No 2nt past end exon 22 Klopp et al (2002) Dec-02
22 Intron g>t - - ? ? Severe No 5nt past end exon 22 Klopp et al (2002) Dec-02
23 Intron G>A - - <1 ? ? ? IVS23 -1 splice mutation Citron et al (2002) Jul-03
23 Intron A>C - - <1 ? Severe ? IVS23 +3 Donor splice mutation Moller-Morlang et al (1999) Oct-99
23 Intron Agta>Aata - - <1 ? Severe No Donor splice mutation Strmecki et al (1999) Oct-99
23 Intron agt>act - - <1 ? Severe No IVS23 +5 Donor splice mutation Vidal et al (2001) Aug-01
24 2213 CAA>CAG Gln Gln ? ? ? ? Possible cryptic splice site McVey et al (unpublished) Dec-96
24 Intron T>A - - 7 ? Mild ? -4 IVS24 acceptor splice site Cutler et al (2002) May-02
24 2173 A/GT > A/TT Ser Ile ? ? Mild No +1 acceptor splice Liu et al (2002) Mar-02
24 Intron AG>AA - - 2 117 Moderate No -1 IVS24 acceptor splice excises exon 25 Gau et al (2003, 2006) Aug-04
25 2223 GTG>ATG Val Met ? ? ? ? +1 Intr24 acceptor splice site Antonarakis et al (unpublished) Dec-94
25 Intron AAG/gt>AAG/ct - - 12 ? Mild No IVS25+1 donor splice site Silva Pinto et al (unpublished) Aug-04
Plasma FVIII:C and/or FVIII:Ag values in this table which do not appear in the indicated publications have been personally communicated.